RNAhub


RNAhub (CWC15_YDR163W_1kb)

CWC15_YDR163W_1kb-b6e0dadc (CWC15_YDR163W_1kb)

The raw output files for each step of the pipeline can be found here.

Input: sequence:

>CWC15 YDR163W SGDID:S000002570, Chromosome IV:781423..781950+/- 1kb
TGCTTCGACCTCCGCAACATCATCAATATCTTCCCATTCATCGTTGTCGTCGTATTCATC
TTCGTTAGTGTTATCAATATTGTTTTTAGGACTAACGCTCTGTGTAATCAAATGTGTCAA
ATAGAAAGGTCGAGCCTTATTCTCCACCTCGTTCTCACCATCTTCCGGCTGTATATAAGA
GACAAACTCCTCCGGTACCAACCCTGTTTTTGATCCTGACTCATTTTCTGCTACTAGCCA
ACCTTGCCCATGCTTGTAACTGATAAATACGATGTCACCCTCAGCCAATCTCAATTCATT
ATCATTTTCGGGTTCGAAATCGTAGAGGGCAACAGCACGTTGGTTAACTATGTAGTCGTC
TGGTAGTGTTATACTTTGTCTTTTATTGTATGTGTCTTCTCCATTTGAAGAATCTGAAAC
CATTTCATCCTCCTCATTGTCTCCATCAAAATAACCATAATGGAGAGGATTCGAATCTGC
GTACGCATAATCTTTGATTGATATATAACCTATTGTGGTTGTTTCTGTATCTTCCATTGT
GTCACTATTTCCTGCCTGCACTGCCTCCACCTCTCTCCCGTTCTGTAATACTGCCTCATT
GAGAACACCTATAGTTTTTTTGAGACCAGATTTCAAAATATTGGTATCTTTTTGTGTGGT
CGTTTCCATCGTTGCCATCGTAGATTCAATAAATATGTCAGTGGAAGTTAAAGTAAGGTA
AAGTTGAGAAAAATACTTAAAATTTGTTAGTATCTATCACGTTTTATTGGTAGCTGTTAT
TATGTGTGATTGAGCGTGTTGTTACTGTGCACTGTGTTGCATTGTCCATTACAAGCTACT
TCTGAAGTGAATTCTCCATTTCTCGCAACTGGAAAAATAAGTTGGTGTGACTAAAAATAA
TAGTCTATCTCTTCACGAAAAGGAAATAGGAATGACAAAGCTCCAAAAGGCTCCATAAAG
AACTTGCTCAGAGGCTGGCATTTGAAAGGGCTACAAGACCATGACCACATCACACAGACC
ACAGTTAGAAGCAAGAAGCGGTGCAAAAGCGGCCGCTTATACACCAACAGGCATCGAGCA
TGCCAGATTACTACCAGGACATACAACATTAAAATACAGGAAATTTAAAGAGGAGGAAAA
TCTTAGAGCAAACTGTGCGCAAGAAGATAGGAGCAACGATAAATCTTTAGAGGAGGCAGT
AATGAACGAAGAGAAACAGGATGTTGTGGGGAGTGGGAACCTCCAAGAAACCCGCAGTGA
GAAAGACCAAAAAGACTCGTTGCAAGAGCTGCTGGTTACTCAAAAAAATAAAGTGGAAGA
TAAAGCAGAGCTTGAAGGAAATGAACAATTAAAGGGAGGAAATTCGTCTAGGCGATCGTG
GAGAAAGGGCACGGCGTTTGGACGCCAT

Database: fungi

PDB hits:

No hits were detected to the PDB database!

Rfam hits:

No hits were detected to the Rfam database!

R-scape on nhmmer alignment:

The final nhmmer alignment can be found here.

Alignments from each iteration: iteration 1, iteration 2, iteration 3.

List of covarying basepairs (#42)
In given structure Left Position Right Position Score E-value p-value Substitutions Power
~ 1790 2286 503.25718 0.00179036 5.89301e-08 47 0.85
~ 1790 1791 458.88630 0.00892263 2.93691e-07 100 0.97
~ 1790 2353 443.17583 0.0157233 5.17538e-07 42 0.83
~ 1826 2343 645.65145 1.01111e-05 3.3281e-10 23 0.64
~ 1828 1830 552.70947 0.000297696 9.79875e-09 222 1.00
~ 1828 2251 512.81159 0.00126656 4.16893e-08 221 1.00
~ 1830 2251 680.83220 2.80678e-06 9.2386e-11 27 0.70
~ 1830 2252 549.54694 0.000334389 1.10065e-08 207 1.00
~ 1830 2354 520.65476 0.000952815 3.13622e-08 80 0.93
~ 1830 2326 471.32771 0.00569025 1.87296e-07 31 0.75
~ 1830 2376 437.41161 0.0193448 6.3674e-07 24 0.66
~ 1842 1844 466.03854 0.00689163 2.2684e-07 420 1.00
~ 1914 1918 496.25343 0.00230706 7.59377e-08 145 1.00
~ 1918 2179 417.35313 0.0398112 1.3104e-06 224 1.00
~ 1918 2173 416.74109 0.0407525 1.34138e-06 333 1.00
~ 2173 2181 534.43578 0.000578566 1.90437e-08 268 1.00
~ 2251 2252 687.78180 2.17822e-06 7.16967e-11 206 1.00
~ 2251 2354 478.21964 0.00443378 1.45939e-07 79 0.93
~ 2251 2376 430.29047 0.0250279 8.238e-07 23 0.64
~ 2251 2326 419.52965 0.0368494 1.21291e-06 30 0.74
~ 2252 2354 458.99423 0.00889047 2.92632e-07 259 1.00
~ 2252 2376 425.68444 0.0295353 9.72165e-07 203 1.00
~ 2252 2326 419.33190 0.0371154 1.22167e-06 210 1.00
~ 2266 2275 1107.58403 4.64158e-13 1.52779e-17 89 0.95
~ 2286 2353 558.29319 0.000243335 8.00944e-09 39 0.81
~ 2286 2315 499.72532 0.00203607 6.70178e-08 70 0.91
~ 2287 2288 871.44994 2.65177e-09 8.72838e-14 168 1.00
~ 2288 2289 454.44914 0.0104778 3.4488e-07 85 0.94
~ 2296 2298 721.76152 6.29805e-07 2.07302e-11 222 1.00
~ 2298 2367 416.66641 0.0408258 1.34379e-06 27 0.70
~ 2307 2308 919.60553 4.54334e-10 1.49546e-14 204 1.00
~ 2313 2315 502.45981 0.00184301 6.06631e-08 98 0.97
~ 2315 2353 547.44670 0.000360891 1.18788e-08 65 0.90
~ 2326 2354 413.48090 0.045802 1.50759e-06 83 0.94
~ 2330 2331 605.04536 4.44431e-05 1.46286e-09 44 0.84
~ 2354 2376 445.32090 0.0145503 4.78927e-07 76 0.93
~ 2358 2359 543.96791 0.000409059 1.34643e-08 200 1.00
~ 2369 2371 496.01777 0.00232805 7.66284e-08 443 1.00
~ 2376 2382 558.27559 0.000243335 8.00944e-09 58 0.89
~ 2503 2521 597.19949 5.91401e-05 1.94661e-09 54 0.88
~ 2560 2585 411.07239 0.0499266 1.64335e-06 324 1.00
~ 2584 2585 507.62597 0.00152927 5.03363e-08 194 1.00
* Base pair in the structure
~ Both residues unpaired in the structure, or no structure is present
' ' At least one residue is involved in other pairing in the structure
BPAIRS expected to covary 39.5 +/- 1.7
BPAIRS observed to covary 42

BPAIRS observed to covary possibly coding 38/42 (0.90%)
Alignment statistics:
Alignment number:    1
Alignment name:      CWC15
Format:              Stockholm
Number of sequences: 1380
Alignment length:    9674
Total # residues:    397816
Smallest:            81
Largest:             1860
Average length:      288.3
Average identity:    48%

R-scape on infernal alignment:

The Infernal alignment can be found here.

List of covarying basepairs (#118)
In given structure Left Position Right Position Score E-value p-value Substitutions Power
* 15 102 223.61873 0.00616169 1.84421e-07 135 1.00
* 108 1917 228.25762 0.00445998 1.33488e-07 91 0.96
* 110 611 255.44498 0.000675423 2.02156e-08 224 1.00
110 1053 204.98376 0.022519 6.74e-07 226 1.00
611 1053 354.21092 6.99403e-07 2.09333e-11 32 0.76
611 1196 291.87533 5.35338e-05 1.60228e-09 211 1.00
611 1924 276.37973 0.00015734 4.70923e-09 95 0.96
611 1782 231.73093 0.00350899 1.05025e-07 38 0.81
~ 617 734 256.99169 0.000606411 1.81501e-08 129 1.00
~ 621 4024 236.98337 0.00243603 7.29109e-08 33 0.77
~ 621 4049 196.20836 0.0413558 1.23779e-06 44 0.84
~ 621 734 195.90382 0.0422266 1.26385e-06 146 1.00
* 622 734 502.89397 2.24651e-11 6.72385e-16 438 1.00
~ 623 734 197.60205 0.0375234 1.12309e-06 128 1.00
734 2128 237.08335 0.00241915 7.24058e-08 150 1.00
~ 738 741 227.54421 0.00469865 1.40632e-07 310 1.00
~ 741 742 309.71990 1.54654e-05 4.62884e-10 113 0.99
~ 745 754 202.59091 0.026605 7.96295e-07 202 1.00
~ 747 753 327.11551 4.60927e-06 1.37957e-10 135 1.00
~ 747 751 310.53443 1.46282e-05 4.37827e-10 85 0.94
~ 747 1009 196.49084 0.0406438 1.21648e-06 133 1.00
~ 753 1009 336.25703 2.43866e-06 7.29896e-11 190 1.00
~ 753 754 282.31907 0.000104019 3.11332e-09 251 1.00
~ 753 1005 222.08077 0.00686256 2.05398e-07 329 1.00
~ 1015 1528 295.28766 4.22587e-05 1.26481e-09 31 0.75
~ 1015 1436 226.29027 0.00512515 1.53397e-07 255 1.00
~ 1022 1034 219.28981 0.00833681 2.49523e-07 12 0.39
~ 1022 1029 212.46238 0.013373 4.00258e-07 128 1.00
~ 1022 1028 202.98832 0.0258759 7.74471e-07 10 0.32
~ 1025 1028 225.55108 0.00538068 1.61045e-07 2 0.00
~ 1025 1029 214.37497 0.0117189 3.50751e-07 120 1.00
~ 1025 2595 205.60793 0.0215247 6.44239e-07 5 0.11
~ 1026 1053 254.05150 0.000741898 2.22052e-08 29 0.73
~ 1026 1035 209.94080 0.0159655 4.77851e-07 14 0.45
~ 1028 1029 213.62827 0.0123459 3.69516e-07 120 1.00
~ 1028 2595 205.14810 0.0222856 6.67012e-07 5 0.11
~ 1029 2595 196.66874 0.0400831 1.1997e-06 123 1.00
~ 1030 1035 230.98815 0.00369679 1.10646e-07 4 0.07
~ 1035 1042 264.31336 0.000363741 1.08869e-08 203 1.00
~ 1035 1053 209.67309 0.0162453 4.86225e-07 19 0.57
~ 1043 1053 224.59740 0.00576798 1.72637e-07 37 0.80
~ 1052 1053 210.37106 0.015474 4.63141e-07 32 0.76
* 1053 1196 509.46404 1.41898e-11 4.24703e-16 213 1.00
1053 1924 252.05787 0.00085259 2.55183e-08 97 0.97
1053 1782 222.25334 0.00676783 2.02563e-07 40 0.82
1196 1924 248.68640 0.00107997 3.23237e-08 276 1.00
1196 1782 240.69689 0.00188352 5.63742e-08 219 1.00
1196 2583 209.02923 0.0169958 5.08688e-07 255 1.00
~ 1202 1530 207.38999 0.0190604 5.70482e-07 28 0.72
* 1209 1315 641.44378 1.4536e-15 4.35067e-20 101 0.97
1315 1772 269.84790 0.000248129 7.42658e-09 106 0.98
1315 1671 224.26547 0.00588951 1.76275e-07 105 0.98
~ 1318 1923 330.95847 3.52614e-06 1.05538e-10 61 0.89
~ 1318 1772 267.66857 0.000288148 8.62433e-09 84 0.94
* 1319 1427 529.41568 3.53858e-12 1.05911e-16 194 1.00
1319 1536 212.11766 0.0137022 4.10112e-07 246 1.00
1427 1428 291.55280 5.46626e-05 1.63607e-09 121 1.00
1427 1536 197.70890 0.0372637 1.11531e-06 164 1.00
~ 1428 1530 199.54827 0.0328839 9.84223e-07 74 0.92
* 1436 1528 410.28274 1.41491e-08 4.23487e-13 246 1.00
1528 2131 209.50597 0.0164155 4.91319e-07 36 0.79
~ 1530 1531 223.33355 0.00629151 1.88307e-07 21 0.61
~ 1531 1533 221.17582 0.00730553 2.18656e-07 26 0.69
* 1536 1666 435.98790 2.36537e-09 7.07963e-14 231 1.00
* 1671 1772 477.60581 1.30271e-10 3.89906e-15 113 0.99
1772 1923 260.02310 0.000490521 1.46814e-08 91 0.96
~ 1780 1783 207.55322 0.0187973 5.62609e-07 15 0.47
* 1785 1914 325.46377 5.16997e-06 1.54739e-10 60 0.89
~ 1915 1919 291.22564 5.60098e-05 1.67639e-09 425 1.00
1917 1919 430.89445 3.37335e-09 1.00965e-13 245 1.00
~ 1919 1923 494.44660 4.04545e-11 1.21081e-15 251 1.00
~ 1919 1925 277.56064 0.000144742 4.33215e-09 496 1.00
~ 1919 1922 220.59789 0.00761661 2.27967e-07 550 1.00
~ 1919 1921 216.52166 0.0100925 3.02072e-07 227 1.00
~ 1919 1920 194.07350 0.0480166 1.43715e-06 221 1.00
~ 1923 2131 268.90615 0.000264156 7.90627e-09 59 0.89
~ 1924 2274 266.82091 0.000305694 9.14951e-09 103 0.98
~ 1924 2583 197.90795 0.0367496 1.09992e-06 139 1.00
* 1926 2123 427.57974 4.24435e-09 1.27034e-13 231 1.00
~ 2125 2132 343.07762 1.51939e-06 4.54758e-11 7 0.20
~ 2126 2132 340.76390 1.78308e-06 5.33681e-11 8 0.24
~ 2126 2587 236.01157 0.00260234 7.78887e-08 5 0.11
~ 2126 2131 231.32407 0.00360793 1.07986e-07 27 0.70
~ 2126 2128 225.52116 0.00539942 1.61606e-07 24 0.66
~ 2126 2129 210.10314 0.0157452 4.71257e-07 30 0.74
~ 2126 2271 206.54017 0.0202199 6.05188e-07 9 0.28
~ 2127 2128 215.36303 0.0109322 3.27204e-07 227 1.00
~ 2128 2132 318.65925 8.29755e-06 2.48348e-10 28 0.72
~ 2128 2129 267.76453 0.000286151 8.56456e-09 50 0.86
~ 2128 2133 223.38547 0.00626969 1.87653e-07 342 1.00
~ 2128 2131 218.24833 0.00896792 2.68412e-07 47 0.85
~ 2128 2130 212.10798 0.0137022 4.10112e-07 217 1.00
~ 2129 2132 214.22656 0.0118417 3.54426e-07 34 0.78
~ 2130 2132 278.08599 0.000139795 4.18409e-09 201 1.00
~ 2131 2132 266.79277 0.000306759 9.18138e-09 31 0.75
~ 2131 2271 197.79627 0.0371345 1.11145e-06 32 0.76
~ 2131 2587 196.53416 0.0405029 1.21226e-06 28 0.72
~ 2132 2271 344.98030 1.33123e-06 3.98442e-11 13 0.42
~ 2132 2269 270.28117 0.000240484 7.19776e-09 163 1.00
~ 2132 2133 268.20151 0.000277334 8.30068e-09 326 1.00
~ 2132 2587 231.40861 0.00358294 1.07238e-07 9 0.28
~ 2132 2274 225.22475 0.00551319 1.65011e-07 29 0.73
~ 2132 2270 220.83847 0.00748542 2.24041e-07 363 1.00
* 2133 2269 306.04302 2.00054e-05 5.98768e-10 477 1.00
2269 2271 199.92690 0.0319827 9.57251e-07 164 1.00
* 2271 2587 407.61583 1.70148e-08 5.09258e-13 10 0.32
2271 2274 383.02358 9.42643e-08 2.82136e-12 30 0.74
2271 2272 246.51261 0.0012541 3.75355e-08 12 0.39
2272 2274 235.67341 0.00266644 7.98071e-08 28 0.72
2272 2587 230.28878 0.00388111 1.16163e-07 8 0.24
* 2272 2586 203.32353 0.0252542 7.55866e-07 8 0.24
* 2274 2583 464.16036 3.32235e-10 9.94389e-15 82 0.94
2274 2587 322.67674 6.2819e-06 1.88019e-10 26 0.69
2274 4023 255.50154 0.000670743 2.00755e-08 192 1.00
2586 2587 207.94480 0.0183457 5.49092e-07 6 0.16
* 2593 4019 475.38059 1.52359e-10 4.56014e-15 49 0.86
~ 4043 4046 195.82738 0.0425209 1.27266e-06 200 1.00
~ 4051 4052 202.22246 0.0272598 8.15894e-07 93 0.96
* Base pair in the structure
~ Both residues unpaired in the structure, or no structure is present
' ' At least one residue is involved in other pairing in the structure
BPAIRS expected to covary 107.2 +/- 3.1
BPAIRS observed to covary 118

BPAIRS observed to covary possibly coding 112/118 (0.95%)

Job log: